James L. Christmann
Professor of Biology & Department Chair
Ph.D. Johns Hopkins University, 1976
Voicemail: (707) 664-3054
Office: Darwin 213
National Institutes of Health, University of California (Davis).
Regulation of Gene Expression.
Currently, research in my lab focuses on the PEX5 genes. These genes code for receptors that participate in the transport of enzymes into peroxisomes. Enzymatic reactions that generate toxic peroxides are sequestered in peroxisomes. These reactions include some that are central to photosynthesis and fat metabolism in plants and most other eukaryotes. Research projects involve the use of recombinant DNA and molecular techniques to study several aspects of these genes. They include:
- the location and sequence of introns in the Nicotiana tabacum genes,
- the relationship of intron location in the genes to domain organization of the protein products (receptors), and
- comparison of the genes in N. tabacum to those of other members of the genus and among other members of the family Solinacae.
Among the techniques employed are PCR, DNA cloning, restriction mapping, blotting, sequencing and computer assisted analysis of the sequence. These projects grew from a collaboration with Dr. John Harada at UC Davis and strong collaboration with his lab continues.
Kragler, F., G. Lametschwandtner, J. Christmann, A. Hartig, and J. J. Harada. 1998. Identification and analysis of the plant peroxisomal targeting signal 1 receptor ntPEX5. Proceedings of the National Academy of Sciences 95:13336-13341.
Close, T. J., J. L. Christmann, and R. L.Rodriguez. 1983. M13 bacteriophage and PUC plasmids containing DNA inserts but still capable of galactosidase a complementation. Gene 23:131-136.
Christmann, J. L. and M. E. Dahmus. 1981. Monoclonal antibodies specific for calf thymus RNA polymerases IIo and IIa. Journal of Biological Chemistry 256: 11798-11803.
Molecular Genetics; Recombinant DNA Laboratory; Introductory Biology; Molecular & Cell Biology.